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Lumbar puncture

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Title: Lumbar puncture  
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Subject: Idiopathic intracranial hypertension, Subarachnoid hemorrhage, Heinrich Quincke, Radionuclide cisternogram, Suboccipital puncture
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Lumbar puncture

Lumbar puncture
Lumbar puncture in a sitting position. The reddish-brown swirls on the patient's back are tincture of iodine (an antiseptic).
ICD-9-CM 03.31

A lumbar puncture (or LP, and colloquially known as a spinal tap) is a diagnostic and at times therapeutic medical procedure. Diagnostically it is used to collect cerebrospinal fluid (CSF) to confirm or exclude conditions such as meningitis and subarachnoid hemorrhage and it may be used in diagnosis of other conditions. Increased intracranial pressure is a contraindication, due to risk of cerebellar tonsils herniation. Under some circumstances, lumbar puncture cannot be performed safely (e.g. a severe bleeding tendency). It is regarded as a safe procedure, but post-dural-puncture headache is common.

The procedure is typically performed under local anesthesia and aseptic technique. A needle is used to access the subarachnoid space and fluid collected. Fluid may be sent for biochemical, microbiological, and cytological analysis.

Lumbar puncture was first introduced in 1891 by the German physician Heinrich Quincke.


  • Indications 1
    • Diagnostic 1.1
    • Therapeutic 1.2
    • Contraindications 1.3
  • Mechanism 2
  • Procedure 3
  • Interpretation 4
    • Pressure determination 4.1
    • Cell count 4.2
    • Microbiology 4.3
    • Chemistry 4.4
  • Adverse effects 5
  • History 6
  • References 7
  • External links 8


The indications of lumbar puncture may be diagnostic[1][2] or therapeutic.[1]


The chief diagnostic indications of lumbar puncture is for collection of cerebrospinal fluid (CSF) and its evaluation to exclude infectious,[1][3] inflammatory[1] and neoplastic diseases[1] affecting the central nervous system. The most common purpose is in case of suspected meningitis,[4] since there is no other reliable tool with which meningitis, a life-threatening but highly treatable condition, can be excluded. A lumbar puncture can also be used to detect whether someone has 'Stage 1' or 'Stage 2' Trypanosoma brucei. Young infants commonly require lumbar puncture as a part of the routine workup for fever without a source, as they have a much higher risk of meningitis than older persons and do not reliably show signs of meningeal irritation (meningismus).[4] In any age group, subarachnoid hemorrhage, hydrocephalus, benign intracranial hypertension and many other diagnoses may be supported or excluded with this test. It may also be used to detect the presence of malignant cells in the CSF, as in carcinomatous meningitis or medulloblastoma. A "negative" tap in the context of a workup for subarachnoid hemorrhage, for example, is constituted by less than 10 red blood cells (RBCs)/mm³. Taps that are considered "positive" have a RBC count of 100/mm³ or more.[5]


Lumbar punctures may also be done to inject medications into the cerebrospinal fluid ("intrathecally"), particularly for spinal anesthesia[6] or chemotherapy.


Lumbar puncture should not be performed in the following situations:

  • Idiopathic (unidentified cause) increased intracranial pressure (ICP)
    • Rationale: lumbar puncture in the presence of increased ICP may cause uncal herniation
    • Exception: therapeutic use of lumbar puncture to reduce ICP, but only if obstruction in e.g. the third ventricle has been ruled out
    • Precaution
      • CT brain is advocated by some, especially in the following situations
        • Age >65
        • Reduced GCS
        • Recent history of seizure
        • Focal neurological signs
        • Abnormal respiratory pattern
        • Hypertension with bradycardia and deteriorating consciousness
      • Ophthalmoscopy for papilledema
  • Bleeding diathesis (relative)
  • Infections
    • Skin infection at puncture site
  • Vertebral deformities (scoliosis or kyphosis), in hands of an inexperienced physician.[7][8]


The brain and spinal cord are enveloped by a layer of fluid, 125-150 ml in total (in adults) which acts as a shock absorber and provides a medium for the transfer of nutrients and waste products. The majority is produced by the choroid plexus in the brain and circulates from there to other areas, before being reabsorbed into the circulation (predominantly by the arachnoid granulations).[9]

The cerebrospinal fluid can be accessed most safely in the lumbar cistern, where the spinal cord has terminated into the cauda equina (below the level of the first or second lumbar vertebrae). The lumbar cistern extends into the sacrum.[9]


Illustration depicting lumbar puncture (spinal tap)
Spinal needles used in lumbar puncture.
Illustration depicting common positions for lumbar puncture procedure.

In performing a lumbar puncture, first the patient is usually placed in a left (or right) lateral position with their neck bent in full flexion and knees bent in full flexion up to their chest, approximating a fetal position as much as possible. It is also possible to have the patient sit on a stool and bend their head and shoulders forward. The area around the lower back is prepared using aseptic technique. Once the appropriate location is palpated, local anaesthetic is infiltrated under the skin and then injected along the intended path of the spinal needle. A spinal needle is inserted between the lumbar vertebrae L3/L4, L4/L5[6] or L5/S1[6] and pushed in until there is a "give" that indicates the needle is past the ligamentum flavum. The needle is again pushed until there is a second 'give' that indicates the needle is now past the dura mater. Since the arachnoid membrane and the dura mater exist in flush contact with one another in the living person's spine (due to fluid pressure from CSF in the subarachnoid space pushing the arachnoid membrane out towards the dura), once the needle has pierced the dura mater it has also traversed the thinner arachnoid membrane and is now in the subarachnoid space. The stylet from the spinal needle is then withdrawn and drops of cerebrospinal fluid are collected. The opening pressure of the cerebrospinal fluid may be taken during this collection by using a simple column manometer. The procedure is ended by withdrawing the needle while placing pressure on the puncture site. The spinal level is so selected to avoid spinal injuries.[6] In the past, the patient would often be asked to lie on their back for at least six hours and be monitored for signs of neurological problems, though there is no scientific evidence that this provides any benefit. The technique described is almost identical to that used in spinal anesthesia, except that spinal anesthesia is more often done with the patient in a seated position.

The upright seated position is advantageous in that there is less distortion of spinal anatomy which allows for easier withdrawal of fluid. It is preferred by some practitioners when a lumbar puncture is performed on an obese patient where having them lie on their side would cause a scoliosis and unreliable anatomical landmarks. On the other hand, opening pressures are notoriously unreliable when measured on a seated patient and therefore the left or right lateral (lying down) position is preferred if an opening pressure needs to be measured.

A study with 132 patients looking into different infant positions with relation to lumbar puncture success rates showed that while the sitting flexed position was as successful as the lateral flexed position in obtaining non-traumatic CSF, CSF for culture, and cell count, there was a higher success rate in obtaining CSF in the first attempt in infants younger than 12 months in the sitting flexed position.[10]

Patient anxiety during the procedure can lead to increased CSF pressure, especially if the person holds their breath, tenses their muscles or flexes their knees too tightly against their chest. Diagnostic analysis of changes in fluid pressure during lumbar puncture procedures requires attention both to the patient's condition during the procedure and to their medical history.

Reinsertion of the stylet may decrease the rate of post lumbar puncture headaches.[8]


Analysis of the cerebrospinal fluid generally includes a cell count and determination of the glucose and protein concentrations. The other analytical studies of cerebrospinal fluid are conducted according to the diagnostic suspicion.[1]

Pressure determination

Lumbar puncture in a child suspected of having meningitis.

Increased CSF pressure can indicate congestive heart failure, cerebral edema, subarachnoid hemorrhage, hypo-osmolality resulting from hemodialysis, meningeal inflammation, purulent meningitis or tuberculous meningitis, hydrocephalus, or pseudotumor cerebri. In the setting of raised pressure (or normal pressure hydrocephalus, where the pressure is normal but there is excessive CSF), lumbar puncture may be therapeutic.

Decreased CSF pressure can indicate complete subarachnoid blockage, leakage of spinal fluid, severe dehydration, hyperosmolality, or circulatory collapse. Significant changes in pressure during the procedure can indicate tumors or spinal blockage resulting in a large pool of CSF, or hydrocephalus associated with large volumes of CSF.

Cell count

The presence of white blood cells in cerebrospinal fluid is called pleocytosis. A small number of monocytes can be normal; the presence of granulocytes is always an abnormal finding. A large number of granulocytes often heralds bacterial meningitis. White cells can also indicate reaction to repeated lumbar punctures, reactions to prior injections of medicines or dyes, central nervous system hemorrhage, leukemia, recent epileptic seizure, or a metastatic tumor. When peripheral blood contaminates the withdrawn CSF, a common procedural complication, white blood cells will be present along with erythrocytes, and their ratio will be the same as that in the peripheral blood.

The finding of erythrophagocytosis,[11] where phagocytosed erythrocytes is observed, signifies haemorrhage into the CSF that preceded the lumbar puncture. Therefore, when erythrocytes are detected in the CSF sample, erythrophagocytosis suggests causes other than a traumatic tap, such as intracranial haemorrhage and haemorrhagic herpetic encephalitis. In which case, further investigations are warranted, including imaging and viral culture.


CSF can be sent to the microbiology lab for various types of smears and cultures to diagnose infections.

  • Gram staining may demonstrate bacteria in bacterial meningitis.
  • Microbiological culture is the gold standard for detecting bacterial meningitis. Bacteria, fungi, and viruses can all be cultured by using different techniques.
  • Polymerase chain reaction (PCR) has been a great advance in the diagnosis of some types of meningitis, such as meningitis from herpesvirus and enterovirus. It has high sensitivity and specificity for many infections of the CNS, is fast, and can be done with small volumes of CSF. Even though testing is expensive, it saves cost of hospitalization.
  • Numerous antibody-mediated tests for CSF are available in some countries: these include rapid tests for antigens of common bacterial pathogens, treponemal titers for the diagnosis of neurosyphilis and Lyme disease, Coccidioides antibody, and others.
  • The India ink test is still used for detection of meningitis caused by Cryptococcus neoformans,[12][13] but the cryptococcal antigen (CrAg) test has a higher sensitivity.[14]


Several substances found in cerebrospinal fluid are available for diagnostic measurement.

  • Glucose is usually present in the CSF; the level is usually about 60% that in the peripheral circulation.[15] A fingerstick or venipuncture at the time of lumbar puncture may therefore be performed to assess peripheral glucose levels and determine a predicted CSF glucose value. Decreased glucose levels[16] can indicate fungal, tuberculous[17] or pyogenic infections; lymphomas; leukemia spreading to the meninges; meningoencephalitic mumps; or hypoglycemia. A glucose level of less than one third of blood glucose levels in association with low CSF lactate levels is typical in hereditary CSF glucose transporter deficiency also known as De Vivo disease.[18]
  • Increased glucose levels in the fluid can indicate diabetes, although the 60% rule still applies.[19][20]
  • Increased levels of glutamine[21] are often involved with hepatic encephalopathies,[22][23] Reye's syndrome,[24][25] hepatic coma, cirrhosis,[23] hypercapnia and depression.[26]
  • Increased levels of lactate can occur the presence of cancer of the CNS, multiple sclerosis, heritable mitochondrial disease, low blood pressure, low serum phosphorus, respiratory alkalosis, idiopathic seizures, traumatic brain injury, cerebral ischemia, brain abscess, hydrocephalus, hypocapnia or bacterial meningitis.[19]
  • The enzyme lactate dehydrogenase can be measured to help distinguish meningitides of bacterial origin, which are often associated with high levels of the enzyme, from those of viral origin in which the enzyme is low or absent.[27]
  • Changes in total protein content of cerebrospinal fluid can result from pathologically increased permeability of the blood-cerebrospinal fluid barrier,[28] obstructions of CSF circulation, meningitis, neurosyphilis, brain abscesses, subarachnoid hemorrhage, polio, collagen disease or Guillain-Barré syndrome, leakage of CSF, increases in intracranial pressure or hyperthyroidism. Very high levels of protein may indicate tuberculous meningitis or spinal block.
  • IgG synthetic rate is calculated from measured IgG and total protein levels; it is elevated in immune disorders such as multiple sclerosis, transverse myelitis, and neuromyelitis optica of Devic. Oligoclonal bands may be detected in CSF but not in serum, suggesting intrathecal antibody production.
Cause Appearance Polymorphonuclear Leukocytes Lymphocytes Protein Glucose
Pyogenic bacterial meningitis Yellowish, turbid Markedly increased Slightly increased or Normal Markedly increased Decreased
Viral meningitis Clear fluid Slightly increased or Normal Markedly increased Slightly increased or Normal Normal
Tuberculous meningitis Yellowish and viscous Slightly increased or Normal Markedly increased Increased Decreased
Fungal meningitis Yellowish and viscous Slightly increased or Normal Markedly increased Slightly increased or Normal Normal or decreased

Adverse effects

Post spinal headache with nausea is the most common complication; it often responds to analgesics and infusion of fluids. It was long taught that this complication can often be prevented by strict maintenance of a supine posture for two hours after the successful puncture; this has not been borne out in modern studies involving large numbers of patients. Merritt's Neurology (10th edition), in the section on lumbar puncture, notes that intravenous caffeine injection is often quite effective in aborting these so-called "spinal headaches." Contact between the side of the lumbar puncture needle and a spinal nerve root can result in anomalous sensations (paresthesia) in a leg during the procedure; this is harmless and patients can be warned about it in advance to minimize their anxiety if it should occur. A headache that is persistent despite a long period of bedrest and occurs only when sitting up may be indicative of a CSF leak from the lumbar puncture site. It can be treated by more bedrest, or by an epidural blood patch, where the patient's own blood is injected back into the site of leakage to cause a clot to form and seal off the leak.

Serious complications of a properly performed lumbar puncture are extremely rare.[1] They include spinal or epidural bleeding, adhesive arachnoiditis and trauma to the spinal cord[6] or spinal nerve roots resulting in weakness or loss of sensation, or even paraplegia. The latter is exceedingly rare, since the level at which the spinal cord ends (normally the inferior border of L1, although it is slightly lower in infants) is several vertebral spaces above the proper location for a lumbar puncture (L3/L4). There are case reports of lumbar puncture resulting in perforation of abnormal dural arterio-venous malformations, resulting in catastrophic epidural hemorrhage; this is exceedingly rare. Failure rates can go up to 28% and therefore there is need for skill and experience.[6]

The procedure is not recommended when epidural infection is present or suspected, when topical infections or dermatological conditions pose a risk of infection at the puncture site or in patients with severe psychosis or neurosis with back pain. Some authorities believe that withdrawal of fluid when initial pressures are abnormal could result in spinal cord compression or cerebral herniation; others believe that such events are merely coincidental in time, occurring independently as a result of the same pathology that the lumbar puncture was performed to diagnose. In any case, computed tomography of the brain is often performed prior to lumbar puncture if an intracranial mass is suspected.

Removal of cerebrospinal fluid resulting in reduced fluid pressure has been shown to correlate with greater reduction of cerebral blood flow among patients with Alzheimer's disease. Its clinical significance is uncertain.


Lumbar puncture, early 20th century.

The first technique for accessing the dural space was described by the London physician Walter Essex Wynter. In 1889 he developed a crude cut down with cannulation in four patients with tuberculous meningitis. The main purpose was the treatment of raised intracranial pressure rather than for diagnosis.[29] The technique for needle lumbar puncture was then introduced by the German physician Heinrich Quincke, who credits Wynter with the earlier discovery; he first reported his experiences at an internal medicine conference in Wiesbaden, Germany, in 1891.[30] He subsequently published a book on the subject.[31][32]

The lumbar puncture procedure was taken to the United States by Arthur H. Wentworth an assistant professor at the Harvard Medical School, based at Children's Hospital. In 1893 he published a long paper on diagnosing cerebrospinal meningitis by examining spinal fluid.[33] However, he was criticized by antivivisectionists for having obtained spinal fluid from children. He was acquitted, but, nevertheless, he was uninvited from the then forming Johns Hopkins School of Medicine, where he would have been the first professor of pediatrics.

Historically lumbar punctures were also employed in the process of performing a pneumoencephalography, a nowadays obsolete X-ray imaging study of the brain that was performed extensively from the 1920s until the advent of modern non-invasive neuroimaging techniques such as MRI and CT in the 1970s. During this quite painful procedure, CSF was replaced with air or some other gas via the lumbar puncture in order to enhance the appearance of certain areas of the brain on plain radiographs.


  1. ^ a b c d e f g Sempere, AP; Berenguer-Ruiz, L; Lezcano-Rodas, M; Mira-Berenguer, F; Waez, M (1–15 October 2007). "Lumbar puncture: its indications, contraindications, complications and technique". Revista de neurologia 45 (7): 433–6.  
  2. ^ Gröschel, K; Schnaudigel, S; Pilgram, SM; Wasser, K; Kastrup, A (January 2008). "The diagnostic lumbar puncture". Deutsche Medizinische Wochenschrift 133 (1–2): 39–41.  
  3. ^ Matata, C; Michael, B; Garner, V; Solomon, T (24–30 October 2012). "Lumbar puncture: diagnosing acute central nervous system infections". Nursing standard (Royal College of Nursing (Great Britain) : 1987) 27 (8): 49–56; quiz 58.  
  4. ^ a b Visintin, C; Mugglestone, MA; Fields, EJ; Jacklin, P; Murphy, MS; Pollard, AJ; Guideline Development, Group; National Institute for Health and Clinical, Excellence (28 June 2010). "Management of bacterial meningitis and meningococcal septicaemia in children and young people: summary of NICE guidance". BMJ (Clinical research ed.) 340: c3209.  
  5. ^ Mann, David (2002). "The role of lumbar puncture in the diagnosis of subarachnoid hemorrhage when computed tomography is unavailable.". Journal of the Canadian Association of Emergency Physicians 4 (2): 102–105. 
  6. ^ a b c d e f López, T; Sánchez, FJ; Garzón, JC; Muriel, C (January 2012). "Spinal anesthesia in pediatric patients". Minerva anestesiologica 78 (1): 78–87.  
  7. ^ Roos KL (March 2003). "Lumbar puncture". Semin Neurol 23 (1): 105–14.  
  8. ^ a b Straus SE, Thorpe KE, Holroyd-Leduc J (October 2006). "How do I perform a lumbar puncture and analyze the results to diagnose bacterial meningitis?". JAMA 296 (16): 2012–22.  
  9. ^ a b Wright, B.L.; Lai, J.T.; Sinclair, A.J. (2012). "Cerebrospinal fluid and lumbar puncture: a practical review". Journal of Neurology 259 (8): 1530–1545.  
  10. ^ Hanson, Amy L.; Ros, Simon; Soprano, Joyce. "Analysis of Infant Lumbar Puncture Success Rates". Pediatric Emergency Care 30 (5): 311–314.  
  11. ^ Harald Kluge (2007). Atlas of CSF cytology. Thieme. pp. 45–46.  
  12. ^ Zerpa, R; Huicho, L; Guillén, A (September 1996). "Modified India ink preparation for Cryptococcus neoformans in cerebrospinal fluid specimens." (PDF). Journal of clinical microbiology 34 (9): 2290–1.  
  13. ^ Shashikala; Kanungo, R; Srinivasan, S; Mathew, R; Kannan, M (Jul–Sep 2004). "Unusual morphological forms of Cryptococcus neoformans in cerebrospinal fluid.". Indian journal of medical microbiology 22 (3): 188–90.  
  14. ^ Antinori, Spinello; Radice, Anna; Galimberti, Laura; Magni, Carlo; Fasan, Marco; Parravicini, Carlo (November 2005). "The role of cryptococcal antigen assay in diagnosis and monitoring of cryptococcal meningitis.". Journal of clinical microbiology 43 (11): 5828–9.  
  15. ^ Nigrovic, Lise E.; Kimia, Amir A.; Shah, Samir S.; Neuman, Mark I. (9 February 2012). "Relationship between Cerebrospinal Fluid Glucose and Serum Glucose". New England Journal of Medicine 366 (6): 576–578.  
  16. ^ Hendry, E (June 1939). "The blood and spinal fluid sugar and chloride content in meningitis". Archives of Disease in Childhood 14 (78): 159–72.  
  17. ^ Gierson, HW; Marx, JI (April 1955). "Tuberculous meningitis: the diagnostic and prognostic significance of spinal fluid sugar and chloride". Annals of internal medicine 42 (4): 902–8.  
  18. ^ De Vivo, Darryl C.; Trifiletti, Rosario R.; Jacobson, Ronald I.; Ronen, Gabriel M.; Behmand, Ramin A.; Harik, Sami I. (5 September 1991). "Defective Glucose Transport across the Blood-Brain Barrier as a Cause of Persistent Hypoglycorrhachia, Seizures, and Developmental Delay". New England Journal of Medicine 325 (10): 703–709.  
  19. ^ a b Leen, Wilhelmina G.; Willemsen, Michèl A.; Wevers, Ron A.; Verbeek, Marcel M.; Mendelson, John E. (5 August 2012). Mendelson, John E, ed. "Cerebrospinal Fluid Glucose and Lactate: Age-Specific Reference Values and Implications for Clinical Practice". PLoS ONE 7 (8): e42745.  
  20. ^ Servo, C; Pitkänen, E (December 1975). "Variation in polyol levels in cerebrospinal fluid and serum in diabetic patients". Diabetologia 11 (6): 575–80.  
  21. ^ "Cerebrospinal Fluid Glutamine". Retrieved 11 August 2013. 
  22. ^ Hourani, Benjamin T.; Hamlin, EM; Reynolds, TB (1 June 1971). "Cerebrospinal Fluid Glutamine as a Measure of Hepatic Encephalopathy". Archives of Internal Medicine 127 (6): 1033–6.  
  23. ^ a b Cascino, A; Cangiano, C; Fiaccadori, F; Ghinelli, F; Merli, M; Pelosi, G; Riggio, O; Rossi Fanelli, F; Sacchini, D; Stortoni, M; Capocaccia, L (September 1982). "Plasma and cerebrospinal fluid amino acid patterns in hepatic encephalopathy". Digestive diseases and sciences 27 (9): 828–32.  
  24. ^ Glasgow, Allen M.; Dhiensiri, Kamnual (June 1974). "Improved Assay for Spinal Fluid Glutamine, and Values for Children with Reye's Syndrome" (PDF). Clinical Chemistry 20 (6): 642–644.  
  25. ^ Watanabe, A; Takei, N; Higashi, T; Shiota, T; Nakatsukasa, H; Fujiwara, M; Sakata, T; Nagashima, H (October 1984). "Glutamic acid and glutamine levels in serum and cerebrospinal fluid in hepatic encephalopathy". Biochemical medicine 32 (2): 225–31.  
  26. ^ Levine, J; Panchalingam, K; Rapoport, A; Gershon, S; McClure, RJ; Pettegrew, JW (1 April 2000). "Increased cerebrospinal fluid glutamine levels in depressed patients". Biological Psychiatry 47 (7): 586–93.  
  27. ^ Jay H. Stein (1998). Internal Medicine. Elsevier Health Sciences. pp. 1408–.  
  28. ^ Reiber, Hansotto (2003). "Proteins in cerebrospinal fluid and blood: Barriers, CSF flow rate and source-related dynamics" (PDF). Restorative Neurology and Neuroscience 21 (3–4): 79–96.  
  29. ^ Wynter W. E. (1891). "Four Cases of Tubercular Meningitis in Which Paracentesis of the Theca Vertebralis Was Performed for the Relief of Fluid Pressure". Lancet 1 (3531): 981–2.  
  30. ^ Quincke HI (1891). Verhandlungen des Congresses für Innere Medizin, Zehnter Congress, Wiesbaden 10. pp. 321–331. 
  31. ^ Quincke HI (1902). Die Technik der Lumbalpunktion. Berlin & Vienna. 
  32. ^ Heinrich Irenaeus Quincke at Who Named It?
  33. ^ Susan E. Lederer (1997). Subjected to Science: Human Experimentation in America Before the Second World War. JHU Press. p. 216.  . Page 62 has a reference to an 1896 publication in Boston Med. Surg. J

External links

  • Ellenby, MS; Tegtmeyer, K; Lai, S; Braner, DA (28 September 2006). "Videos in clinical medicine. Lumbar puncture". The New England Journal of Medicine 355 (13): e12.  
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