Global Gene Expression Profiling in Whole-Blood Samples from Individuals Exposed to Metal Fumes

By Wang, Zhaoxi

Book Id: WPLBN0000020111
Format Type: PDF eBook:
File Size: 1.19 MB
Reproduction Date: 2005

Title:	Global Gene Expression Profiling in Whole-Blood Samples from Individuals Exposed to Metal Fumes
Author:	Wang, Zhaoxi
Volume:
Language:	English
Subject:	Government publications, United Nations., United Nations. Office for Disarmament Affairs
Collections:	Government Library Collection, Disarmament Documents
Historic Publication Date:
Publisher:	United Nations- Office for Disarmament Affairs (Unoda)


Citation APA MLA Chicago Wang, B. Z. (n.d.). Global Gene Expression Profiling in Whole-Blood Samples from Individuals Exposed to Metal Fumes. Retrieved from https://self.gutenberg.org/

Description
Government Reference Publication

Excerpt
Excerpt: Accumulating evidence demonstrates that particulate air pollutants can cause both pulmonary and airway inflammation. However, few data show that particulates can induce systemic inflammatory responses. We conducted an exploratory study using microarray techniques to analyze wholeblood total RNA in boilermakers before and after occupational exposure to metal fumes. A selfcontrolled study design was used to overcome the problems of larger between-individual variation interferences with observations of relatively smaller changes caused by environmental exposure. Moreover, we incorporated the dichotomous data of absolute gene expression status in the microarray analyses. Compared with nonexposed controls, we observed that genes with altered expression in response to particulate exposure were clustered in biologic processes related to inflammatory response, oxidative stress, intracellular signal transduction, cell cycle, and programmed cell death. In particular, the preinflammatory cytokine interleukin 8 and one of its receptors, chemokine receptor 4, seemed to play important roles in early-stage response to heavy metal exposure and were down-regulated. Furthermore, most observed expression variations were from nonsmoking exposed individuals, suggesting that smoking profoundly affects whole-blood expression profiles. Our study is the first to demonstrate that with a paired sampling study design of pre- and postexposed individuals, small changes in gene expression profiling can be measured in whole-blood total RNA from a population-based study. This technique can be applied to evaluate the host response to other forms of environmental exposures. Key words: functional pathway, gene expression profiling, inflammation, occupational particulate exposure, whole-blood total RNA. [22 November 2004].