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Bacillus subtilis

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Title: Bacillus subtilis  
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Bacillus subtilis

Bacillus subtilis
TEM micrograph of a B. subtilis cell in cross-section (scale bar = 200 nm)
Scientific classification
Domain: Bacteria
Phylum: Firmicutes
Class: Bacilli
Order: Bacillales
Family: Bacillaceae
Genus: Bacillus
Species: B. subtilis
Binomial name
Bacillus subtilis
(Ehrenberg 1835)
Cohn 1872
  • Vibrio subtilis
  • Until 2008 Bacillus globigii was thought to be B. subtilis but is since formally recognized as Bacillus atrophaeus.[1][2]

Bacillus subtilis, known also as the hay bacillus or grass bacillus, is a model organism to study bacterial chromosome replication and cell differentiation. It is one of the bacterial champions in secreted enzyme production and used on an industrial scale by biotechnology companies.


  • Description 1
  • Habitat 2
  • Reproduction 3
  • Chromosomal replication 4
  • Genome 5
  • Transformation 6
  • Uses 7
    • 1900s 7.1
    • 2000s 7.2
  • Safety 8
    • In Humans 8.1
    • In Animals 8.2
  • See also 9
  • References 10
  • External links 11


Bacillus subtilis is a Gram-positive equivalent of Escherichia coli, an extensively studied Gram-negative bacterium.


This species is commonly found in the upper layers of the soil, and evidence exists that B. subtilis is a normal gut commensal in humans. A 2009 study compared the density of spores found in soil (about 106 spores per gram) to that found in human feces (about 104 spores per gram). The number of spores found in the human gut was too high to be attributed solely to consumption through food contamination.[8]


Sporulating B. subtilis.
Another endospore stain of B. subtilis.

B. subtilis can divide symmetrically to make two daughter cells (binary fission), or asymmetrically, producing a single motile by producing flagella, take up DNA from the environment, or produce antibiotics. These responses are viewed as attempts to seek out nutrients by seeking a more favourable environment, enabling the cell to make use of new beneficial genetic material or simply by killing of competition.

Under stressful conditions, such as nutrient deprivation, B. subtilis undergoes the process of sporulation to ensure the survival of the species. This process has been very well studied and has served as a model organism for studying sporulation.

Chromosomal replication

B. subtilis is a oriC). Replication proceeds bidirectionally and two replication forks progress in clockwise and counterclockwise directions along the chromosome. Chromosome replication is completed when the forks reach the terminus region, which is positioned opposite to the origin on the chromosome map. The terminus region contains several short DNA sequences (Ter sites) that promote replication arrest. Specific proteins mediate all the steps in DNA replication. Comparison between the proteins involved in chromosomal DNA replication in B. subtilis and in Escherichia coli reveals similarities and differences. Although the basic components promoting initiation, elongation, and termination of replication are well-conserved, some important differences can be found (such as one bacterium missing proteins essential in the other). These differences underline the diversity in the mechanisms and strategies that various bacterial species have adopted to carry out the duplication of their genomes.[9]


B. subtilis has about 4,100 genes. Of these, only 192 were shown to be indispensable; another 79 were predicted to be essential, as well. A vast majority of essential genes were categorized in relatively few domains of cell metabolism, with about half involved in information processing, one-fifth involved in the synthesis of cell envelope and the determination of cell shape and division, and one-tenth related to cell energetics.[10]

The complete genome sequence of B. subtilis sub-strain QB928 has 4,146,839 DNA base pairs and 4,292 genes. The QB928 strain is widely used in genetic studies due to the presence of various markers [aroI(aroK)906 purE1 dal(alrA)1 trpC2].[11]

Several noncoding RNAs have been characterized in the B. subtilis genome in 2009, including Bsr RNAs.[12] Microarray-based comparative genomic analyses have revealed that B. subtilis members show considerable genomic diversity.[13]


Natural bacterial transformation involves the transfer of DNA from one bacterium to another through the surrounding medium. In B. subtilis, length of transferred DNA is greater than 1271 kb (more than 1 million bases).[14] The transferred DNA is likely double-stranded DNA and is often more than a third of the total chromosome length of 4215 kb.[15] It appears that about 7-9% of the recipient cells take up an entire chromosome.[16]

In order for a recipient bacterium to bind, take up exogenous DNA from another bacterium of the same species and recombine it into its chromosome, it must enter a special physiological state called competence. Competence in B. subtilis is induced toward the end of logarithmic growth, especially under conditions of amino-acid limitation.[17] Under these stressful conditions of semistarvation, cells typically have just one copy of their chromosome and likely have increased DNA damage. To test whether transformation is an adaptive function for B. subtilis to repair its DNA damage, experiments were conducted using UV light as the damaging agent.[18][19][20] These experiments led to the conclusion that competence, with uptake of DNA, is specifically induced by DNA-damaging conditions, and that transformation functions as a process for recombinational repair of DNA damage.[21]



Gram-stained B. subtilis

Cultures of B. subtilis were popular worldwide before the introduction of antibiotics as an immunostimulatory agent to aid treatment of gastrointestinal and urinary tract diseases. It was used throughout the 1950s as an alternative medicine, which upon digestion has been found to significantly stimulate broad-spectrum immune activity including activation of secretion of specific antibodies galenic forms like tablets, capsules, and powder.

Since the 1960s B. subtilis has had a history as a test species in spaceflight experimentation. Its endospores can survive up to 6 years in space if coated by dust particles protecting it from solar UV rays.[25]* It has been used as an extremophile survival indicator in outer space such as Exobiology Radiation Assembly,[26][27] EXOSTACK,[28][29] and EXPOSE orbital missions.[30][31][32]

Wild-type natural isolates of B. subtilis are difficult to work with compared to laboratory strains that have undergone domestication processes of mutagenesis and selection. These strains often have improved capabilities of transformation (uptake and integration of environmental DNA), growth, and loss of abilities needed "in the wild". And, while dozens of different strains fitting this description exist, the strain designated '168' is the most widely used.

Colonies of B. subtilis grown on a culture dish in a molecular biology laboratory

B. globigii, a closely related but phylogenetically distinct species now known as Bacillus atrophaeus [33][34] was used as a biowarfare simulant during Project SHAD (aka Project 112).[35] Subsequent genomic analysis showed that the strains used in those studies were products of deliberate enrichment for strains that exhibited abnormally high rates of sporulation.[36]

A strain of B. subtilis formerly known as Bacillus natto is used in the commercial production of the Japanese food natto, as well as the similar Korean food cheonggukjang.


  • As a model organism, B. subtilis is commonly used in laboratory studies directed at discovering the fundamental properties and characteristics of Gram-positive spore-forming bacteria.[37] In particular, the basic principles and mechanisms underlying formation of the durable endospore have been deduced from studies of spore formation in B. subtilis.
  • It can convert some explosives into harmless compounds of nitrogen, carbon dioxide, and water.
  • Its surface-binding properties play a role in safe radionuclide waste [e.g. thorium (IV) and plutonium (IV)] disposal.
  • Recombinant strains pBE2C1 and pBE2C1AB were used in production of polyhydroxyalkanoates (PHA), and malt waste can be used as their carbon source for lower-cost PHA production.
  • Due to its excellent fermentation properties, with high product yields (20 to 25 gram per litre) it is used to produce various enzymes, such as amylase and proteases.[38]
  • Other enzymes produced by B. subtilis and B. licheniformis are widely used as additives in laundry detergents.
  • It is used to produce hyaluronic acid, which is used in the joint-care sector in healthcare [39] and cosmetics.
  • B. subtilis is used as a soil inoculant in horticulture and agriculture.[40][41][42]
  • It may provide some benefit to saffron growers by speeding corm growth and increasing stigma biomass yield.[43]
  • Monsanto has isolated a gene from B. subtilis that expresses cold shock protein B and spliced it into their drought-tolerant corn hybrid MON 87460, which was approved for sale in the US in November 2011.[44][45]
  • It is used as an "indicator organism" during gas sterilization procedures, to ensure a sterilization cycle has completed successfully.[46][47] This is due to the difficulty in sterilizing endospores.
  • Novel strains of B. subtilis that could use 4-fluorotryptophan (4FTrp) but not canonical tryptophan (Trp) for propagation were isolated. As Trp is only coded by a single codon, there is evidence that Trp can be displaced by 4FTrp in the genetic code. The experiments showed that the canonical genetic code can be mutable.[48]


In Humans

B. subtilis is only known to cause disease in severely immunocompromised patients, and can conversely be used as a probiotic in healthy individuals.[49] It rarely causes food poisoning.[50] Some B. subtilis strains produce the proteolytic enzyme subtilisin.

B. subtilis spores can survive the extreme heat during cooking. Some B. subtilis strains are responsible for causing ropiness — a sticky, stringy consistency caused by bacterial production of long-chain polysaccharides — in spoiled bread dough. For a long time, bread ropiness was associated uniquely with B. subtilis species by biochemical tests. Molecular assays (randomly amplified polymorphic DNA PCR assay, denaturing gradient gel electrophoresis analysis, and sequencing of the V3 region of 16S ribosomal DNA) revealed greater Bacillus species variety in ropy breads, which all seems to have a positive amylase activity and high heat resistance.[51]

B. subtilis and substances derived from it has been evaluated by different authoritative bodies for their safe and beneficial use in food. In the United States, an opinion letter issued in the early 1960s by the

  • WikiSubti "up-to-date information for all genes of Bacillus subtilis"
  • Bacillus subtilis Final Risk Assessment on
  • genome browserBacillus subtilis

External links

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See also

B. subtilis was reviewed by the FDA Canadian Food Inspection Agency Animal Health and Production Feed Section has classified Bacillus culture dehydrated approved feed ingredients as a silage additive under Schedule IV-Part 2-Class 8.6 and assigned the International Feed Ingredient number IFN 8-19-119.

In Animals

B. subtilis has been granted "Qualified Presumption of Safety" status by the European Food Safety Authority.[54] B. subtilis is part of the authoritative list of microorganisms with a documented history of safe use in food, established by the International Dairy Federation in collaboration with the European Food and Feed Cultures Association in 2002, and updated in 2012.

[53] as effective for preservation of health.Ministry of Health, Labour and Welfare natto as its principal component are FOSHU (Foods for Specified Health Use) approved by the Japanese B. subtilis. The natto product and the B. subtilis has not been implicated in adverse events potentially attributable to the presence of natto intake; during this long history of widespread use, 2 viable cells per gram. The fermented beans are recognized for their contribution to a healthy gut flora and vitamin K8, which is commonly consumed in Japan, and contains as many as 10Natto are widely available and have been safely used in a variety of food applications. This includes consumption of Japanese fermented soy bean, in the form of B. subtilis strain were in common use in food prior to January 1, 1958, and that nontoxigenic and nonpathogenic strains of B. subtilis The FDA stated the enzymes derived from the [52]

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