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World Health Organization : Year 2005 ; The Special Programme for Research and Training in Tropical Diseases Irm Diag Den, No. 05.1: Dengue Diagnostics ; Proceedings of a Joint TDR/World Health Organization and Pdvi Workshop

By World Health Organization

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Book Id: WPLBN0000180484
Format Type: PDF eBook
File Size: 2.1 MB
Reproduction Date: 2005
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Title: World Health Organization : Year 2005 ; The Special Programme for Research and Training in Tropical Diseases Irm Diag Den, No. 05.1: Dengue Diagnostics ; Proceedings of a Joint TDR/World Health Organization and Pdvi Workshop  
Author: World Health Organization
Volume:
Language: English
Subject: Health., Public health, Wellness programs
Collections: Medical Library Collection, World Health Collection
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Publication Date:
Publisher: World Health Organization

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Organization, W. H. (n.d.). World Health Organization : Year 2005 ; The Special Programme for Research and Training in Tropical Diseases Irm Diag Den, No. 05.1. Retrieved from http://self.gutenberg.org/


Description
Medical Reference Publication

Excerpt
Executive summary Introduction: the diagnostic challenge Dengue is diagnosed by isolation of the virus, by serology, or by molecular diagnostic methods. Although several commercial kits for the diagnosis of dengue are available, concerns have arisen with respect to the performance characteristics of these kits. In theory, it should be possible to establish a diagnosis of on-going or recent dengue infection during the acute or early convalescent stages of the disease. When such tests require the identification of the virus or the viral genome they are expensive and require specialized laboratories. Affordable commercial kits of adequate sensitivity and specificity that are able to diagnose dengue infection during the acute stage have not been developed. It is also possible that virological or physiological tests applied during the acute stage of the illness might be capable of predicting the severity of dengue virus infection.

Table of Contents
Table of contents Abbreviations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Executive summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Introduction: the diagnostic challenge . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Summary of discussion of tests for acute infection . . . . . . . . . . . . . . . . . . . . . . 3 Summary of tests discussed for secondary infection . . . . . . . . . . . . . . . . . . . . 4 Rapid diagnostic tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Strategies for consideration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Establishment of reference laboratories . . . . . . . . . . . . . . . . . . . . . . . . 7 Research needs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Project organization and funding . . . . . . . . . . . . . . . . . . . . . . . . 8 Next steps . . . . . . . . . . . . . . . . . . . . . . . . 9 Goals of the workshop . . . . . . . . . . . . . . . . . . . . . . . . 11 Laboratory diagnosis of dengue . . . . . . . . . . . . . . . . . . . . . . . . 15 Immunological responses to dengue infections . . . . . . . . . . . . . . . . . . . . . . . 16 Testing for dengue: an overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Laboratory diagnosis of dengue infection: epidemiology and field studies . . . . . . . 20 Laboratory diagnosis of dengue using commercial kits . . . . . . . . . . . . . . . 29 Commercial kits and reagents . . . . . . . . . . . . . . . . . . . . . . . . 30 Diagnostic accuracy of rapid immunochromatographic assays for the detection of IgM antibodies to dengue virus during the acute phase of infection: a systematic review and meta-analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 Diagnostic needs for dengue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 Detection of NS1 from dengue virus: basis for early diagnosis and a prognostic marker of disease progression (1) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Detection of NS1 from dengue virus: basis for early diagnosis and a prognostic marker of disease progression (2) . . . . . . . . . . . . . . . . . . . . . . . . 43 Detection of antibodies to dengue: neutralization . . . . . . . . . . . . . . . . . . . . . . . 44 Antibodies against dengue viruses: problems of interpretation when more than one flavivirus co-circulates in a community . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 The IgM-capture ELISA using VLPs or virus-infected mouse brain antigens to detect IgM antibody in serum from patients with evidence of flaviviral infection . . . . . . . . . 52 Problems encountered in the molecular detection of dengue viruses . . . . . . . . . . . . 60 Chips and other new technologies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67 Rapid detection and differentiation of dengue virus serotypes by RT-LAMP . . . . 68

 

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