Examination of Adduct Formation in Vivo in the Mouse between Benzo(A)Pyrene and Dna of Skin and Hemoglobin of Red Blood Cells

By Kao, John

Book Id: WPLBN0000098316
Format Type: PDF eBook
File Size: 0.2 MB
Reproduction Date: 2005

Title:	Examination of Adduct Formation in Vivo in the Mouse between Benzo(A)Pyrene and Dna of Skin and Hemoglobin of Red Blood Cells
Author:	Kao, John
Volume:
Language:	English
Subject:	Government publications, United Nations., United Nations. Office for Disarmament Affairs
Collections:	Government Library Collection, Disarmament Documents
Historic Publication Date:
Publisher:	United Nations- Office for Disarmament Affairs (Unoda)


Citation APA MLA Chicago Kao, J. (n.d.). Examination of Adduct Formation in Vivo in the Mouse between Benzo(A)Pyrene and Dna of Skin and Hemoglobin of Red Blood Cells. Retrieved from http://self.gutenberg.org/

Description
Government Reference Publication

Excerpt
Excerpt: Many chemical carcinogens, among them the polycyclic aromatic hydrocarbons, have been shown to bind co\dently to cellular nacromoleculesa fter they undergo netabolic activation (1-3). Furthermore, the tumorigenic and carcinogenic activities of these compounds, and in particular benzo(a)pyrene (BaP) have been correlated with their ability to form adducts with DNA (4,5). We are interested in devising techniques which will allow us to detect and quantitate exposure to chemical carcinogens and which eventually can be used in risk analysis with humans. Ow recent research with HPLCI fluorescence has demonstrated that we can detect, identify, and quantitate the binding of BaP with DNA of mouse skin (6-8). The technique not only allows femtomole amounts of BaPDE associated with DNA isolated from a single mouse skin to be detected using conventional instrumentation, but also establishes the stereochemical origin of the adduct.